nuclear protein isolation kit Search Results


minutetm plasma membrane isolation kit  (Invent Biotechnologies)
96
Invent Biotechnologies minutetm plasma membrane isolation kit
Minutetm Plasma Membrane Isolation Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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adipocytes  (Invent Biotechnologies)
93
Invent Biotechnologies adipocytes
Adipocytes, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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minutetm nuclear envelope protein extraction kit  (Invent Biotechnologies)
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Invent Biotechnologies minutetm nuclear envelope protein extraction kit
Minutetm Nuclear Envelope Protein Extraction Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neuronal tissue  (Invent Biotechnologies)
94
Invent Biotechnologies neuronal tissue
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plant cytosolic  (Invent Biotechnologies)
94
Invent Biotechnologies plant cytosolic
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Plant Cytosolic, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear extraction kit  (Invent Biotechnologies)
92
Invent Biotechnologies nuclear extraction kit
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Nuclear Extraction Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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commercial assay or kit cell nuclear/ cytoplasm protein isolation kit  (Beyotime)
90
Beyotime commercial assay or kit cell nuclear/ cytoplasm protein isolation kit
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Commercial Assay Or Kit Cell Nuclear/ Cytoplasm Protein Isolation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear protein isolation kit  (Beyotime)
90
Beyotime nuclear protein isolation kit
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Nuclear Protein Isolation Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear protein isolation kit - by Bioz Stars, 2026-03
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nuclear protein extraction isolation (fractionation-translocation) kit  (FIVEphoton Biochemicals)
90
FIVEphoton Biochemicals nuclear protein extraction isolation (fractionation-translocation) kit
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Nuclear Protein Extraction Isolation (Fractionation Translocation) Kit, supplied by FIVEphoton Biochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear protein isolation–translocation assay kit  (Beyotime)
90
Beyotime nuclear protein isolation–translocation assay kit
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Nuclear Protein Isolation–Translocation Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear and mitochondrial protein isolation kit  (Qiagen)
90
Qiagen nuclear and mitochondrial protein isolation kit
(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a <t>cytosolic</t> marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).
Nuclear And Mitochondrial Protein Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a cytosolic marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).

Journal: bioRxiv

Article Title: Phosphorus Availability Modulates Flowering Time Through Subcellular Reprogramming of bGLU25 and GRP7 in Flowering Plants

doi: 10.1101/2025.01.02.631137

Figure Lengend Snippet: (A) Yeast Two-Hybrid (Y2H) analysis of an A. thaliana shoot library identified JACALIN-LECTIN LIKE1 (AtJAC1) as an interactor of both the full-length bGLU25 and ΔbGLU25. This is shown by growth on selective media (S, DO-3) lacking tryptophan, leucine, and histidine. Positive controls included SMAD (Suppressor of Mothers against Decapentaplegic) and SMURF (SMad Ubiquitination Regulatory Factors) from the human TGF-β (Transforming Growth Factor-β) /Smad pathway, while negative controls were bGLU25, ΔbGLU25 or AtJAC1 alone. (B) The BiFC assay tested GRP7-mVenus n interaction with bGLU25-mVenus c or ΔbGLU25-mVenus c in N. benthamiana , using GRP7 and AtJAC1 interaction as positive controls (scale bar = 10 µm). (C) Transient expression of GRP7 in N. benthamiana leaves alongside a cytosolic marker, cRFP, under varying P conditions (+P and -P). Under -P conditions, GRP7 is mainly found in the cytosol (scale bar = 10 μm). C, cytosol. N, Nucleus. (D) Flowering time data for various genotypes grown under +P conditions, including wild type (Col-0), ΔbGLU25-2 , a complemented bglu25-1 line with bGLU25 (bGLU25-C2), and others. Results are presented as means ± SD (n = 18), with letters indicating significant differences (p < 0.05). (E) Flowering time data for the same genotypes grown under -P conditions, with means ± SD (n = 18) and letters indicate significant differences (p < 0.05).

Article Snippet: To separate cytosolic, ER-enriched, or nuclear fractions, Arabidopsis plants were homogenized and fractionated using either the Minute™ Plant Microsomal Membrane Extraction Kit (Invent Biotechnologies, MM-018) or the Minute™ Plant Cytosolic and Nuclear Protein Isolation Kit (Invent Biotechnologies, PF-045), following the manufacturer’s instructions.

Techniques: Bimolecular Fluorescence Complementation Assay, Expressing, Marker